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TOP2A ELISA Kit (Mouse)

CAT: 0247-OKEH07192-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07192-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Topoisomerase (DNA) II alpha
Gene Aliases
DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, alpha isozyme; Top; Top-2.
Gene ID
21973
Accession Number
NP_035753
Reactivity
Mouse, Mus musculus
Target
DNA topoisomerase 2-alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Top2a ELISA Kit (Mouse) (OKEH07192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Top2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Top2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Top2a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 110%
Sensitivity
39.1 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse DNA topoisomerase 2-alpha
NCBI Gene Symbol
Top2a
Protein Name
Q01320
Gene Name URL
Top2a
Nucleotide Accession Number
NM_011623

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