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STAT6 ELISA Kit (Human)

CAT: 0247-OKEH07175-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH07175-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Signal transducer and activator of transcription 6
Gene Aliases
D12S1644; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT.
Gene ID
6778
Accession Number
NP_001171549
Reactivity
Homo sapiens, Human
Target
Signal transducer and activator of transcription 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology STAT6 ELISA Kit (Human) (OKEH07175) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-STAT6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated STAT6 and incubated. The STAT6 found in the sample or standards competes with the biotinylated STAT6 for limited binding sites on the immobilized anti-STAT6 antibody. Excess unbound biotinylated STAT6 and sample or standard STAT6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated STAT6 captured in the well and inversely proportional to the amount of STAT6 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into matrix = 109.0%
Sensitivity
0.811 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Signal transducer and activator of transcription 6
NCBI Gene Symbol
STAT6
Protein Name
P42226
Gene Name URL
STAT6
Nucleotide Accession Number
NM_001178078

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