STAT6 ELISA Kit (Human)

• Reproducibility:

  Inter-Assay CV

• Gene Name:

  Signal transducer and activator of transcription 6

• Gene Aliases:

  D12S1644; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT.

• Gene ID:

  6778

• Accession Number:

  NP_001171549

• Reactivity:

  Homo sapiens, Human

• Target:

  Signal transducer and activator of transcription 6

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology STAT6 ELISA Kit (Human) (OKEH07175) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-STAT6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated STAT6 and incubated. The STAT6 found in the sample or standards competes with the biotinylated STAT6 for limited binding sites on the immobilized anti-STAT6 antibody. Excess unbound biotinylated STAT6 and sample or standard STAT6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated STAT6 captured in the well and inversely proportional to the amount of STAT6 which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  1.56-100ng/mL

• Recovery:

  Mean recovery when spiking into matrix = 109.0%

• Sensitivity:

  0.811 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Signal transducer and activator of transcription 6

• NCBI Gene Symbol:

  STAT6

• Protein Name:

  P42226

• Gene Name URL:

  STAT6

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001178078