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SPON2 ELISA Kit (Mouse)

CAT: 0247-OKEH07171-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07171-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Spondin 2, extracellular matrix protein
Gene Aliases
2310045I24Rik; AI504350; Mi; Mindin; M-s; Mspondin; M-spondin; spondin-2.
Gene ID
100689
Accession Number
NP_598664
Reactivity
Mouse, Mus musculus
Target
Spondin-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Spon2 ELISA Kit (Mouse) (OKEH07171) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Spon2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Spon2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Spon2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into matrix = 101.7%
Sensitivity
15.79 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Spondin-2
NCBI Gene Symbol
Spon2
Protein Name
Q8BMS2
Gene Name URL
Spon2
Nucleotide Accession Number
NM_133903

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