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RRM1 ELISA Kit (Mouse)

CAT: 0247-OKEH07139-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07139-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Ribonucleotide reductase M1
Gene Aliases
Ribonucleoside-diphosphate reductase large subunit; ribonucleoside-diphosphate reductase subunit M1; ribonucleotide reductase large subunit; Rn; RnrM1.
Gene ID
20133
Accession Number
NP_033129
Reactivity
Mouse, Mus musculus
Target
Ribonucleoside-diphosphate reductase large subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Rrm1 ELISA Kit (Mouse) (OKEH07139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rrm1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rrm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rrm1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 88.1%
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Ribonucleoside-diphosphate reductase large subunit
NCBI Gene Symbol
Rrm1
Protein Name
P07742
Gene Name URL
Rrm1
Nucleotide Accession Number
NM_009103

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