PLA2G2A ELISA Kit (Mouse)

CAT: 0247-OKEH07109-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07109-96WSize:96 Tests
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Phospholipase A2, group IIA (platelets, synovial fluid)
Gene Aliases
EF; enhancing factor; GIIC sPLA2; group IIA phospholipase A2; modifier of Min1; Mom1; non-pancreatic secreted type II phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, membrane associated; Pla2; secretory group II phospholipase A2; sPLA2; sPla2-IIA.
Gene ID
18780
Accession Number
NP_001076000
Reactivity
Mouse, Mus musculus
Target
Phospholipase A2, membrane associated
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pla2g2a ELISA Kit (Mouse) (OKEH07109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pla2g2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pla2g2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pla2g2a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into matrix = 101%
Sensitivity
0.319 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phospholipase A2, membrane associated
NCBI Gene Symbol
Pla2g2a
Protein Name
P31482
Gene Name URL
Pla2g2a
Nucleotide Accession Number
NM_001082531

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