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OXTR ELISA Kit (Human)

CAT: 0247-OKEH07098-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07098-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Oxytocin receptor
Gene Aliases
OTR; OT-R; oxytocin receptor.
Gene ID
5021
Accession Number
NP_000907
Reactivity
Homo sapiens, Human
Target
Oxytocin receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology OXTR ELISA Kit (Human) (OKEH07098) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-OXTR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated OXTR and incubated. The OXTR found in the sample or standards competes with the biotinylated OXTR for limited binding sites on the immobilized anti-OXTR antibody. Excess unbound biotinylated OXTR and sample or standard OXTR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated OXTR captured in the well and inversely proportional to the amount of OXTR which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 94%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Oxytocin receptor
NCBI Gene Symbol
OXTR
Protein Name
P30559
Gene Name URL
OXTR
Nucleotide Accession Number
NM_000916

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