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OSM ELISA Kit (Rat)

CAT: 0247-OKEH07096-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07096-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Oncostatin M
Gene Aliases
Oncostatin-M.
Gene ID
289747
Accession Number
NP_001006962
Reactivity
Rat, Rattus norvegicus
Target
Oncostatin-M
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Osm ELISA Kit (Rat) (OKEH07096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Osm has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Osm is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Osm captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into matrix = 92%
Sensitivity
15.76 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Oncostatin-M
NCBI Gene Symbol
Osm
Protein Name
Q65Z15
Gene Name URL
Osm
Nucleotide Accession Number
NM_001006961

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