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NR5A2 ELISA Kit (Rat)

CAT: 0247-OKEH07092-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07092-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Nuclear receptor subfamily 5, group A, member 2
Gene Aliases
Fetoprotein transcription factor; Ftf; FTZ-F1 beta; FTZ-F1 beta2 protein; liver receptor homolog 1; Lrh-1; nuclear receptor subfamily 5 group A member 2.
Gene ID
60349
Accession Number
NP_068510
Reactivity
Rat, Rattus norvegicus
Target
Nuclear receptor subfamily 5 group A member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nr5a2 ELISA Kit (Rat) (OKEH07092) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nr5a2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nr5a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nr5a2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 102%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Nuclear receptor subfamily 5 group A member 2
NCBI Gene Symbol
Nr5a2
Protein Name
Q9QWM1
Gene Name URL
Nr5a2
Nucleotide Accession Number
NM_021742

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