MYH4 ELISA Kit (Human)

• Reproducibility:

  Inter-Assay CV

• Gene Name:

  Myosin heavy chain 4

• Gene Aliases:

  MYH2B; MyHC-2B; MyHC-IIb; myosin heavy chain 2b; Myosin heavy chain 4; myosin heavy chain IIb; myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; myosin-4.

• Gene ID:

  4622

• Accession Number:

  NP_060003

• Reactivity:

  Homo sapiens, Human

• Target:

  Myosin-4

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology MYH4 ELISA Kit (Human) (OKEH07077) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-MYH4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MYH4 and incubated. The MYH4 found in the sample or standards competes with the biotinylated MYH4 for limited binding sites on the immobilized anti-MYH4 antibody. Excess unbound biotinylated MYH4 and sample or standard MYH4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MYH4 captured in the well and inversely proportional to the amount of MYH4 which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into matrix = 92.9%

• Sensitivity:

  0.159 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Myosin-4

• NCBI Gene Symbol:

  MYH4

• Protein Name:

  Q9Y623

• Gene Name URL:

  MYH4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_017533