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LYVE1 ELISA Kit (Human)

CAT: 0247-OKEH07052-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07052-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Lymphatic vessel endothelial hyaluronan receptor 1
Gene Aliases
Cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP-1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; HAR; hyaluronic acid receptor; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; XLKD1.
Gene ID
10894
Accession Number
NP_006682
Reactivity
Homo sapiens, Human
Target
Lymphatic vessel endothelial hyaluronic acid receptor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LYVE1 ELISA Kit (Human) (OKEH07052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LYVE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LYVE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LYVE1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 106%
Sensitivity
39.9 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Lymphatic vessel endothelial hyaluronic acid receptor 1
NCBI Gene Symbol
LYVE1
Protein Name
Q9Y5Y7
Gene Name URL
LYVE1
Nucleotide Accession Number
NM_006691

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