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KRT18 ELISA Kit (Rat)

CAT: 0247-OKEH07028-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07028-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Keratin 18
Gene Aliases
CK-18; cytokeratin-18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; keratin, type I cytoskeletal 18; Keratin-18; Krt1-18.
Gene ID
294853
Accession Number
NP_446428
Reactivity
Rat, Rattus norvegicus
Target
Keratin, type I cytoskeletal 18
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Krt18 ELISA Kit (Rat) (OKEH07028) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt18 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Krt18 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt18 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into matrix = 87%
Sensitivity
0.317 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Keratin, type I cytoskeletal 18
NCBI Gene Symbol
Krt18
Protein Name
Q5BJY9
Gene Name URL
Krt18
Nucleotide Accession Number
NM_053976

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