HSPA5 ELISA Kit (Mouse)

• Reproducibility:

  Inter-Assay CV

• Gene Name:

  Heat shock protein 5

• Gene Aliases:

  78 kDa glucose-regulated protein;78kD; AL022860; AU019543; B; baffl; baffled; binding-immunoglobulin protein; Bip; D2Wsu141; D2Wsu141e; D2Wsu17; D2Wsu17e; endoplasmic reticulum chaperone BiP; glucose regulated protein, 78 kDa; Grp; Grp78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD) ; heat shock protein 70 family protein 5; heat shock protein family A member 5; Hsce7; Hsce70; HSP70 family protein 5; immunoglobulin heavy chain-binding protein; mB; mBiP; Sez; Sez7; SEZ-7; XAP-1 antigen.

• Gene ID:

  14828

• Accession Number:

  NP_001156906

• Reactivity:

  Mouse, Mus musculus

• Target:

  Endoplasmic reticulum chaperone BiP

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Hspa5 ELISA Kit (Mouse) (OKEH07000) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa5 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  78-5000pg/mL

• Recovery:

  Mean recovery when spiking into matrix = 96%

• Sensitivity:

  39.9 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse 78 kDa glucose-regulated protein

• NCBI Gene Symbol:

  Hspa5

• Protein Name:

  P20029

• Gene Name URL:

  Hspa5

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001163434