HSPA5 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Heat shock protein 5

78 kDa glucose-regulated protein;78kD; AL022860; AU019543; B; baffl; baffled; binding-immunoglobulin protein; Bip; D2Wsu141; D2Wsu141e; D2Wsu17; D2Wsu17e; endoplasmic reticulum chaperone BiP; glucose regulated protein, 78 kDa; Grp; Grp78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD) ; heat shock protein 70 family protein 5; heat shock protein family A member 5; Hsce7; Hsce70; HSP70 family protein 5; immunoglobulin heavy chain-binding protein; mB; mBiP; Sez; Sez7; SEZ-7; XAP-1 antigen.

14828

NP_001156906.1

Mouse|Mus musculus

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965) . Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443) . Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity) . In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity) . Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 5 (HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock 70kDa Protein 5 (HSPA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

0.156-10ng/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 EDTA Plasma (n=5) 86-101 95 Heparin Plasma (n=5) 80-97 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 Serum (n=5) 97-104 101 EDTA Plasma (n=5) 86-101 95 EDTA Plasma (n=5) 86-101 95 Heparin Plasma (n=5) 80-97 93 Heparin Plasma (n=5) 80-97 93

0.057 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-HSPA5 Microplate 96 Wells (12 x 8 Well strips) HSPA5 Lyophilized Standard 2 100X Biotinylated HSPA5 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-HSPA5 Microplate 96 Wells (12 x 8 Well strips) Anti-HSPA5 Microplate 96 Wells (12 x 8 Well strips) HSPA5 Lyophilized Standard 2 HSPA5 Lyophilized Standard 2 100X Biotinylated HSPA5 Detector Antibody 1 x 120 uL 100X Biotinylated HSPA5 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5) . No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.

655

Hspa5

Endoplasmic reticulum chaperone BiP

Hspa5