HSD11B1 ELISA Kit (Rat)

• Reproducibility:

  Inter-Assay CV

• Gene Name:

  Hydroxysteroid 11-beta dehydrogenase 1

• Gene Aliases:

  11beta-HSD1;11-beta-HSD1;11-beta-hydroxysteroid dehydrogenase 1;11-beta-hydroxysteroid dehydrogenase, type 1;11-DH; corticosteroid 11-beta-dehydrogenase isozyme 1; cortocosteroid 11-beta dehydrogenase; hydroxysteroid dehydrogenase, 11 beta type 1; LRRGT00065.

• Gene ID:

  25116

• Accession Number:

  NP_058776

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  Corticosteroid 11-beta-dehydrogenase isozyme 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Hsd11b1 ELISA Kit (Rat) (OKEH06996) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsd11b1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsd11b1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsd11b1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into matrix = 106%

• Sensitivity:

  0.188 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Rat Corticosteroid 11-beta-dehydrogenase isozyme 1

• NCBI Gene Symbol:

  Hsd11b1

• Protein Name:

  P16232

• Gene Name URL:

  Hsd11b1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_017080