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EDIL3 ELISA Kit (Human)

CAT: 0247-OKEH06952-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06952-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
EGF like repeats and discoidin domains 3
Gene Aliases
DEL1; developmental endothelial locus-1; developmentally-regulated endothelial cell locus 1 protein; EGF-like repeat and discoidin I-like domain-containing protein 3; EGF-like repeats and discoidin I-like domains 3; integrin-binding protein DEL1.
Gene ID
10085
Accession Number
NP_001265571
Reactivity
Homo sapiens, Human
Target
EGF-like repeat and discoidin I-like domain-containing protein 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EDIL3 ELISA Kit (Human) (OKEH06952) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDIL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDIL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EDIL3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 82%
Sensitivity
40.4 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human EGF-like repeat and discoidin I-like domain-containing protein 3
NCBI Gene Symbol
EDIL3
Protein Name
O43854
Gene Name URL
EDIL3
Nucleotide Accession Number
NM_001278642

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