DPYSL3 ELISA Kit (Human)

Inter-Assay CV

7732-18-5

Dihydropyrimidinase like 3

Collapsin response mediator protein 4; collapsin response mediator protein 4 long; CRMP4; CRMP-4; dihydropyrimidinase-related protein 3; DRP3; DRP-3; LCRMP; testicular secretory protein Li 7; ULIP; ULIP-1; unc-33-like phosphoprotein 1.

1809

NP_001184223

Homo sapiens, Human

Dihydropyrimidinase-related protein 3

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Aviva Systems Biology DPYSL3 ELISA Kit (Human) (OKEH06950) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-DPYSL3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DPYSL3 and incubated. The DPYSL3 found in the sample or standards competes with the biotinylated DPYSL3 for limited binding sites on the immobilized anti-DPYSL3 antibody. Excess unbound biotinylated DPYSL3 and sample or standard DPYSL3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DPYSL3 captured in the well and inversely proportional to the amount of DPYSL3 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates

0.625-40ng/mL

Mean recovery when spiking into matrix = 86.9%

0.328 ng/mL

Store as indicated in product manual.

Natural and recombinant Human Dihydropyrimidinase-related protein 3

DPYSL3

Q14195

DPYSL3