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DNMT1 ELISA Kit (Mouse)

CAT: 0247-OKEH06944-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06944-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
DNA methyltransferase (cytosine-5) 1
Gene Aliases
Cxxc9; DNA (cytosine-5) -methyltransferase 1; DNA methyltransferase MmuI; DNA MTase MmuI; Dnmt; Dnmt1o; m.MmuI; MCMT; Met1; Met-1; MommeD; MommeD2; MTa; MTase.
Gene ID
13433
Accession Number
NP_001186360
Reactivity
Mouse, Mus musculus
Target
DNA (cytosine-5) -methyltransferase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dnmt1 ELISA Kit (Mouse) (OKEH06944) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dnmt1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dnmt1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dnmt1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 92%
Sensitivity
0.165 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse DNA (cytosine-5) -methyltransferase 1
NCBI Gene Symbol
Dnmt1
Protein Name
P13864
Gene Name URL
Dnmt1
Nucleotide Accession Number
NM_001199431

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