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COL15A1 ELISA Kit (Mouse)

CAT: 0247-OKEH06910-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06910-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Collagen, type XV, alpha 1
Gene Aliases
Collagen alpha-1 (XV) chain; procollagen type XV; type XV collagen.
Gene ID
12819
Accession Number
NP_034058
Reactivity
Mouse, Mus musculus
Target
Collagen alpha-1 (XV) chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Col15a1 ELISA Kit (Mouse) (OKEH06910) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Col15a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Col15a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Col15a1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into matrix = 102%
Sensitivity
39.9 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Collagen alpha-1 (XV) chain
NCBI Gene Symbol
Col15a1
Protein Name
O35206
Gene Name URL
Col15a1
Nucleotide Accession Number
NM_009928

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