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CD38 ELISA Kit (Rat)

CAT: 0247-OKEH06892-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06892-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
CD38 molecule
Gene Aliases
2'-phospho-ADP-ribosyl cyclase;2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase;2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase) ; CD38H; cyclic ADP-ribose hydrolase 1.
Gene ID
25668
Accession Number
NP_037259
Reactivity
Rat, Rattus norvegicus
Target
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd38 ELISA Kit (Rat) (OKEH06892) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd38 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd38 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd38 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into matrix = 110%
Sensitivity
1.67 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
NCBI Gene Symbol
Cd38
Protein Name
Q64244
Gene Name URL
Cd38
Nucleotide Accession Number
NM_013127

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