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CARNS1 ELISA Kit (Mouse)

CAT: 0247-OKEH06880-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06880-96WSize:96 Well
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Carnosine synthase 1
Gene Aliases
AI790298; Atpg; Atpgd1; ATP-grasp domain containing 1; ATP-grasp domain-containing protein 1; carnosine synthase 1; mKIAA1394.
Gene ID
107239
Accession Number
XP_006531682
Reactivity
Mouse, Mus musculus
Target
Carnosine synthase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Carns1 ELISA Kit (Mouse) (OKEH06880) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Carns1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Carns1 and incubated. The Carns1 found in the sample or standards competes with the biotinylated Carns1 for limited binding sites on the immobilized anti-Carns1 antibody. Excess unbound biotinylated Carns1 and sample or standard Carns1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Carns1 captured in the well and inversely proportional to the amount of Carns1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 95.7%
Sensitivity
0.438 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Carnosine synthase 1
NCBI Gene Symbol
Carns1
Protein Name
Q6ZPS2
Gene Name URL
Carns1
Nucleotide Accession Number
XM_006531619

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