CAMK2A ELISA Kit (Mouse)

Inter-Assay CV

7732-18-5

Calcium/calmodulin-dependent protein kinase II alpha

Alpha-CaMKII; calcium/calmodulin-dependent protein kinase type II subunit alpha; caM kinase II subunit alpha; CaMK II; CaMKII; caMK-II subunit alpha; mKIAA0968; R74975.

12322

NP_001273738

Mouse, Mus musculus

Calcium/calmodulin-dependent protein kinase type II subunit alpha

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Camk2a ELISA Kit (Mouse) (OKEH06875) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Camk2a antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Camk2a and incubated. The Camk2a found in the sample or standards competes with the biotinylated Camk2a for limited binding sites on the immobilized anti-Camk2a antibody. Excess unbound biotinylated Camk2a and sample or standard Camk2a is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Camk2a captured in the well and inversely proportional to the amount of Camk2a which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates

0.78-50ng/mL

Mean recovery when spiking into matrix = 96.6%

0.39 ng/mL

Store as indicated in product manual.

Natural and recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha

Camk2a

P11798

Camk2a