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BID ELISA Kit (Mouse)

CAT: 0247-OKEH06864-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06864-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
BH3 interacting domain death agonist
Gene Aliases
2700049M22Rik; AI875481; AU022477; BH3-interacting domain death agonist; p22 BID.
Gene ID
12122
Accession Number
NP_031570
Reactivity
Mouse, Mus musculus
Target
BH3-interacting domain death agonist
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Bid ELISA Kit (Mouse) (OKEH06864) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bid has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bid is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bid captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 100.3%
Sensitivity
0.443 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse BH3-interacting domain death agonist
NCBI Gene Symbol
Bid
Protein Name
P70444
Gene Name URL
Bid
Nucleotide Accession Number
NM_007544

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