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ACTN1 ELISA Kit (Mouse)

CAT: 0247-OKEH06840-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06840-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Actinin, alpha 1
Gene Aliases
3110023F10Rik; Actn1a; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; alpha-actinin-1; F-actin cross-linking protein; non-muscle alpha-actinin-1.
Gene ID
109711
Accession Number
NP_598917
Reactivity
Mouse, Mus musculus
Target
Alpha-actinin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Actn1 ELISA Kit (Mouse) (OKEH06840) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Actn1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Actn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Actn1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 85.1%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Alpha-actinin-1
NCBI Gene Symbol
Actn1
Protein Name
Q7TPR4
Gene Name URL
Actn1
Nucleotide Accession Number
NM_134156

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