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ACAT1 ELISA Kit (Mouse)

CAT: 0247-OKEH06835-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06835-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Acetyl-Coenzyme A acetyltransferase 1
Gene Aliases
6330585C21Rik; Ac; Acat; acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase, mitochondrial.
Gene ID
110446
Accession Number
NP_659033
Reactivity
Mouse, Mus musculus
Target
Acetyl-CoA acetyltransferase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Acat1 ELISA Kit (Mouse) (OKEH06835) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Acat1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Acat1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Acat1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Detection Range
7.8-500pg/mL
Sensitivity
4.18 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Acetyl-CoA acetyltransferase, mitochondrial
NCBI Gene Symbol
Acat1
Protein Name
Q8QZT1
Gene Name URL
Acat1
Nucleotide Accession Number
NM_144784

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