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IFIT1 ELISA Kit (Mouse)

CAT: 0247-OKEH06734-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH06734-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Reproducibility CV% < 7.3% (n=20) < br>Inter-Assay Reproducibility CV% < 9.4% (n=24)
CAS Number
7732-18-5
Gene Name
Interferon-induced protein with tetratricopeptide repeats 1
Gene Aliases
GARG-16; glucocorticoid-attenuated response gene 16 protein; Ifi; Ifi56; IFI-56K; interferon-induced 56 kDa protein; interferon-induced protein with tetratricopeptide repeats 1; ISG; ISG56; P56.
Gene ID
15957
Accession Number
NP_032357
Reactivity
Mouse, Mus musculus
Target
Interferon-induced protein with tetratricopeptide repeats 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ifit1 ELISA Kit (Mouse) (OKEH06734) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ifit1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ifit1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ifit1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into serum = 102%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Ifit1
Protein Name
Q64282
Gene Name URL
Ifit1
Nucleotide Accession Number
NM_008331

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