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NT5C3B ELISA Kit (Chicken)

CAT: 0247-OKEH06654-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06654-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
5'-nucleotidase, cytosolic IIIB
Gene Aliases
5'-nucleotidase, cytosolic III-like;7-methylguanosine nucleotidase;7-methylguanosine phosphate-specific 5'-nucleotidase; cN-III-like protein; cytosolic 5'-nucleotidase 3B; cytosolic 5'-nucleotidase III-like protein; N (7) -methylguanylate 5'-phosphatase; NT5C3L.
Gene ID
420034
Accession Number
NP_001006334
Reactivity
Chicken, Gallus gallus
Target
7-methylguanosine phosphate-specific 5'-nucleotidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Nt5c3b ELISA Kit (Chicken) (OKEH06654) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5c3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nt5c3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5c3b captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/ml
Sensitivity
0.29 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken Cytosolic 5'-nucleotidase III-like protein
NCBI Gene Symbol
NT5C3B
Protein Name
Q5ZKF6
Gene Name URL
NT5C3B
Nucleotide Accession Number
NM_001006334

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