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PMP2 ELISA Kit (Pig)

CAT: 0247-OKEH06617-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06617-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Peripheral myelin protein 2
Gene Aliases
Myelin P2 protein.
Gene ID
100151890
Accession Number
NP_001171994
Reactivity
Porcine, Sus scrofa
Target
Myelin P2 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pmp2 ELISA Kit (Pig) (OKEH06617) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pmp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pmp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pmp2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/ml
Sensitivity
0.099 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig Myelin P2 protein
NCBI Gene Symbol
PMP2
Protein Name
P86412
Gene Name URL
PMP2
Nucleotide Accession Number
NM_001185065

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