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CRYGD ELISA Kit (Mouse)

CAT: 0247-OKEH06497-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06497-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Crystallin, gamma D
Gene Aliases
Aey; Aey4; Cryg-; Cryg-1; DGcry-; DGcry-1; gamma-crystallin 1; gamma-crystallin D; gamma-D-crystallin; lens opacity 12; Lop12.
Gene ID
12967
Accession Number
NP_001077372
Reactivity
Mouse, Mus musculus
Target
Gamma-crystallin D
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Crygd ELISA Kit (Mouse) (OKEH06497) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Crygd antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crygd and incubated. The Crygd found in the sample or standards competes with the biotinylated Crygd for limited binding sites on the immobilized anti-Crygd antibody. Excess unbound biotinylated Crygd and sample or standard Crygd is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crygd captured in the well and inversely proportional to the amount of Crygd which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105.9%
Sensitivity
0.426 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Gamma-crystallin D
NCBI Gene Symbol
Crygd
Protein Name
P04342
Gene Name URL
Crygd
Nucleotide Accession Number
NM_001083903

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