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CKB ELISA Kit (Mouse)

CAT: 0247-OKEH06448-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06448-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Creatine kinase, brain
Gene Aliases
Bck; B-CK; Ck3; Ck-3; Ckbb; CPK-B; creatine kinase B chain; creatine kinase B-type; creatine phosphokinase B-type.
Gene ID
12709
Accession Number
NP_660295
Reactivity
Mouse, Mus musculus
Target
Creatine kinase B-type
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ckb ELISA Kit (Mouse) (OKEH06448) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ckb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ckb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ckb captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.189 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Creatine kinase B-type
NCBI Gene Symbol
Ckb
Protein Name
Q04447
Gene Name URL
Ckb
Nucleotide Accession Number
NM_145252

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