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SLC5A2 ELISA Kit (Rat)

CAT: 0247-OKEH06381-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06381-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Solute carrier family 5 member 2
Gene Aliases
Low affinity Na-dependent glucose transporter (SGLT2) ; low affinity sodium-glucose cotransporter; Na (+) /glucose cotransporter 2; Sglt2; sodium/glucose cotransporter 2; solute carrier family 5 (sodium/glucose cotransporter), member 2; Solute carrier family 5 member 2.
Gene ID
64522
Accession Number
NP_006059
Reactivity
Rat, Rattus norvegicus
Target
Sodium/glucose cotransporter 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc5a2 ELISA Kit (Rat) (OKEH06381) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc5a2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc5a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc5a2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 104%
Sensitivity
0.163 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Sodium/glucose cotransporter 2
NCBI Gene Symbol
Slc5a2
Protein Name
P53792
Gene Name URL
Slc5a2
Nucleotide Accession Number
NM_006068

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