PDGFC ELISA Kit (Mouse)
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PDGFC ELISA Kit (Mouse)
Gene Name :
Platelet-derived growth factor, C polypeptideGene Aliases :
1110064L01Rik; AI647969; fallotein; PDGF-; PDGF-C; platelet-derived growth factor C; SCDGF; spinal cord-derived growth factor; VEGF-E.Gene ID :
54635Accession Number :
NP_076463Reactivity :
Mouse, Mus musculusTarget :
Platelet-derived growth factor CType :
ELISA KitApplications :
Enzyme-linked Immunosorbent assay-SandwichAssay Principle :
Aviva Systems Biology Pdgfc ELISA Kit (Mouse) (OKEH06376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfc captured in the well.Assay Protocol :
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type :
Serum, plasma and other biological fluidsDetection Range :
1.56-100ng/mLSensitivity :
0.85 ng/mLReconstitution :
Store as indicated in product manual.Specificity :
Natural and recombinant Mouse Platelet-derived growth factor CNCBI Gene Symbol :
PdgfcProtein Name :
Q8CI19Gene Name URL :
PdgfcCAS Number :
7732-18-5Nucleotide Accession Number :
NM_023973

