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PCNA ELISA Kit (Rat)

CAT: 0247-OKEH06276-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06276-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Proliferating cell nuclear antigen
Gene Aliases
Cyclin; Pcna/cyclin; PCNAR; proliferating cell nuclear antigen.
Gene ID
25737
Accession Number
NP_071776.1
Reactivity
Rat, Rattus norvegicus
Target
Proliferating cell nuclear antigen
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pcna ELISA Kit (Rat) (OKEH06276) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pcna has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pcna is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pcna captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Proliferating cell nuclear antigen
Protein Length
261
NCBI Gene Symbol
Pcna
Protein Name
P04961
Gene Name URL
Pcna
Nucleotide Accession Number
NM_022381.3

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