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LTC4S ELISA Kit (Rat)

CAT: 0247-OKEH06257-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH06257-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Leukotriene C4 synthase
Gene Aliases
Glutathione S-transferase LTC4; leukotriene C4 synthase; leukotriene-C (4) synthase; LTC4 synthase.
Gene ID
114097
Accession Number
NP_446091.2
Reactivity
Rat, Rattus norvegicus
Target
Leukotriene C4 synthase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ltc4s ELISA Kit (Rat) (OKEH06257) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ltc4s has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ltc4s is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ltc4s captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 92%
Sensitivity
15.63 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Leukotriene C4 synthase
Protein Length
150
NCBI Gene Symbol
Ltc4s
Protein Name
Q925U2
Gene Name URL
Ltc4s
Nucleotide Accession Number
NM_053639.2

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