ACTA2 ELISA Kit (Rat)

CAT: 0247-OKEH06186-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH06186-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Actin alpha 2, smooth muscle
Gene Aliases
Actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; alpha-actin-2; smooth muscle alpha-actin.
Gene ID
81633
Accession Number
NP_112266.1
Reactivity
Rat, Rattus norvegicus
Target
Actin, aortic smooth muscle
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Acta2 ELISA Kit (Rat) (OKEH06186) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Acta2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Acta2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Acta2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 96%
Sensitivity
1.56 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Actin, aortic smooth muscle
Protein Length
377
NCBI Gene Symbol
Acta2
Protein Name
P62738
Gene Name URL
Acta2
Nucleotide Accession Number
NM_031004.2

Related Products

CatalogName
OKEH03339-96WACTA2 ELISA Kit (Mouse)
OKEH00958-96WACTA2 ELISA Kit (Human)
OAAF08240-100ULACTA2/ACTG2 Antibody
OAAF08240-50ULACTA2/ACTG2 Antibody
OKEH03339ACTA2 ELISA Kit (Mouse) (OKEH03339)
OKEH00958ACTA2 ELISA Kit (Human) : 96 Wells (OKEH00958)
OAAF08241-100ULOAAF08241-100UL - ACTC1/ACTA1/ACTA2/ACTG2/ACTB/ACTG1 Antibody
OAAF08241-50ULOAAF08241-50UL - ACTC1/ACTA1/ACTA2/ACTG2/ACTB/ACTG1 Antibody
OAAN00928-50ULACTA2 Antibody
OAAN02191-200ULACTA2 Antibody

Alternative Products

CatalogName
E074530Rat ACTa2 ELISA Kit
EK20673Rat Actin, aortic smooth muscle (ACTA2) ELISA Kit
EK19583Rat Acta2/Actin, aortic smooth muscle ELISA Kit
ELK9039-01Rat ACTa2 (Actin Alpha 2, Smooth Muscle) ELISA Kit
YP-W36121-01Rat Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit
RK03464Rat Actin Alpha 2, Smooth Muscle ELISA Kit (ACTa2)
JL21054-01Rat Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit
ELK9039MS-01Rat ACTa2 (Actin Alpha 2, Smooth Muscle) Microsample ELISA Kit
EKU08517Human/Mouse/Rat Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit
353662-01ACTA2 (Actin Alpha 2, Smooth Muscle) BioAssayTM ELISA Kit (Rat) discontinued

Popular Products