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KRT2 ELISA Kit (Rat)

CAT: 0247-OKEH06174-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06174-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Keratin 2
Gene Aliases
CK 2e; CK-2e; cytokeratin-2e; epithelial keratin-2e; K2e; Kb2; keratin 2, type II; keratin, type II cytoskeletal 2 epidermal; keratin-2 epidermis; keratin-2e; type II keratin Kb2; Type-II keratin Kb2.
Gene ID
406228
Accession Number
NP_001008899.1
Reactivity
Rat, Rattus norvegicus
Target
Keratin, type II cytoskeletal 2 epidermal
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Krt2 ELISA Kit (Rat) (OKEH06174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Krt2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 108%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Keratin, type II cytoskeletal 2 epidermal
Protein Length
685
NCBI Gene Symbol
Krt2
Protein Name
Q6IG02
Gene Name URL
Krt2
Nucleotide Accession Number
NM_001008899.1

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