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HSD17B13 ELISA Kit (Rat)

CAT: 0247-OKEH06103-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06103-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Hydroxysteroid (17-beta) dehydrogenase 13
Gene Aliases
17-beta-HSD 13;17-beta-hydroxysteroid dehydrogenase 13; short-chain dehydrogenase/reductase 9.
Gene ID
305150
Accession Number
NP_001009684.1
Reactivity
Rat, Rattus norvegicus
Target
17-beta-hydroxysteroid dehydrogenase 13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hsd17b13 ELISA Kit (Rat) (OKEH06103) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsd17b13 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hsd17b13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsd17b13 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 92%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat 17-beta-hydroxysteroid dehydrogenase 13
Protein Length
300
NCBI Gene Symbol
Hsd17b13
Protein Name
Q5M875
Gene Name URL
Hsd17b13
Nucleotide Accession Number
NM_001009684.1

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