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ESM1 ELISA Kit (Rat)

CAT: 0247-OKEH06056-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH06056-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Endothelial cell-specific molecule 1
Gene Aliases
Endothelial cell-specific molecule 1; ESM-1 secretory protein; Pg25; pineal specific PG25 protein.
Gene ID
64536
Accession Number
NP_072126.1
Reactivity
Rat, Rattus norvegicus
Target
Endothelial cell-specific molecule 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Esm1 ELISA Kit (Rat) (OKEH06056) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Esm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Esm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Esm1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
15.71 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Endothelial cell-specific molecule 1
Protein Length
184
NCBI Gene Symbol
Esm1
Protein Name
P97682
Gene Name URL
Esm1
Nucleotide Accession Number
NM_022604.2

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