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EFHD2 ELISA Kit (Rat)

CAT: 0247-OKEH05972-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05972-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
EF-hand domain family, member D2
Gene Aliases
EF-hand domain-containing protein D2, Swiprosin-1, Sws1
Gene ID
298609
Accession Number
NP_001026818.1
Reactivity
Rat
Target
May regulate B-cell receptor (BCR) -induced immature and primary B-cell apoptosis. Plays a role as negative regulator of the canonical NF-kappa-B-activating branch. Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Efhd2 ELISA Kit (Rat) (OKEH05972) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Efhd2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Efhd2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Efhd2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 108%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat EF-hand domain-containing protein D2
Protein Length
239
NCBI Gene Symbol
EFHD2
Protein Name
EF-hand domain-containing protein D2
Gene Name URL
EFHD2
Nucleotide Accession Number
NM_001031648.1

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