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C8B ELISA Kit (Rat)

CAT: 0247-OKEH05957-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05957-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Complement C8 beta chain
Gene Aliases
Complement component 8 subunit beta; complement component 8, beta polypeptide; complement component 8, beta subunit; complement component C8 beta chain.
Gene ID
313421
Accession Number
NP_001178688.1
Reactivity
Rat, Rattus norvegicus
Target
Complement component C8 beta chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology C8b ELISA Kit (Rat) (OKEH05957) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C8b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for C8b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C8b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.8-500ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 108%
Sensitivity
4.1 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Complement component C8 beta chain
Protein Length
589
NCBI Gene Symbol
C8b
Protein Name
P55314
Gene Name URL
C8b
Nucleotide Accession Number
NM_001191759.1

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