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CORO1A ELISA Kit (Mouse)

CAT: 0247-OKEH05925-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05925-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Coronin, actin binding protein 1A
Gene Aliases
Cl; Clabp; clipin-A; coronin 1; coronin-1A; coronin-like actin binding protein; coronin-like protein A; coronin-like protein p57; Lmb; Lmb3; p5; p57; TACO; tryptophan aspartate-containing coat protein.
Gene ID
12721
Accession Number
NP_001288303.1
Reactivity
Mouse, Mus musculus
Target
Coronin-1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Coro1a ELISA Kit (Mouse) (OKEH05925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Coro1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Coro1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Coro1a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 106.3%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Coronin-1A
Protein Length
461
NCBI Gene Symbol
Coro1a
Protein Name
O89053
Gene Name URL
Coro1a
Nucleotide Accession Number
NM_001301374.1

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