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NT5E ELISA Kit (Mouse)

CAT: 0247-OKEH05923-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05923-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
5' nucleotidase, ecto
Gene Aliases
2210401F01Rik;5'-NT;5'-nucleotidase; AI447961; CD73; ecto-5'-nucleotidase; eNT; NT; Nt5.
Gene ID
23959
Accession Number
NP_035981.1
Reactivity
Mouse, Mus musculus
Target
5'-nucleotidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nt5e ELISA Kit (Mouse) (OKEH05923) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5e has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nt5e is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5e captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86.6%
Sensitivity
41.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse 5'-nucleotidase
Protein Length
576
NCBI Gene Symbol
Nt5e
Protein Name
Q61503
Gene Name URL
Nt5e
Nucleotide Accession Number
NM_011851.4

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