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KIF5A ELISA Kit (Mouse)

CAT: 0247-OKEH05916-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05916-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Kinesin family member 5A
Gene Aliases
D10Bwg0738e; K; Khc; Kif; Kif5; kinesin heavy chain; kinesin heavy chain isoform 5A; kinesin heavy chain neuron-specific 1; Kns; mKIAA4086; neuronal kinesin heavy chain; NKHC.
Gene ID
16572
Accession Number
NP_001034089.1
Reactivity
Mouse, Mus musculus
Target
Kinesin heavy chain isoform 5A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Kif5a ELISA Kit (Mouse) (OKEH05916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kif5a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Kif5a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kif5a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90.6%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Kinesin heavy chain isoform 5A
Protein Length
1027
NCBI Gene Symbol
Kif5a
Protein Name
P33175
Gene Name URL
Kif5a
Nucleotide Accession Number
NM_001039000.4

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