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UBR4 ELISA Kit (Mouse)

CAT: 0247-OKEH05909-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05909-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Ubiquitin protein ligase E3 component n-recognin 4
Gene Aliases
1810009A16Rik; A930005E13Rik; D930005K06Rik; E3 ubiquitin-protein ligase UBR4; Gm1032; Gm1666; mKIAA0462; N28143; N-recognin-4; p60; p600; RBAF600; retinoblastoma-associated factor 600; RING-type E3 ubiquitin transferase UBR4; zinc finger UBR1-type protein 1; zinc finger, UBR1 type 1; Zubr; Zubr1.
Gene ID
69116
Accession Number
NP_001153791.1
Reactivity
Mouse, Mus musculus
Target
E3 ubiquitin-protein ligase UBR4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ubr4 ELISA Kit (Mouse) (OKEH05909) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ubr4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ubr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ubr4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 106.2%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse E3 ubiquitin-protein ligase UBR4
Protein Length
5180
NCBI Gene Symbol
Ubr4
Protein Name
A2AN08
Gene Name URL
Ubr4
Nucleotide Accession Number
NM_001160319.1

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