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ANXA3 ELISA Kit (Mouse)

CAT: 0247-OKEH05858-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05858-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Annexin A3
Gene Aliases
35-alpha calcimedin; An; annexin A3; annexin III; annexin-3; Anx3; lipocortin III; PAP-III; placental anticoagulant protein III.
Gene ID
11745
Accession Number
NP_038498.2
Reactivity
Mouse, Mus musculus
Target
Annexin A3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Anxa3 ELISA Kit (Mouse) (OKEH05858) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Anxa3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 103.7%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Annexin A3
Protein Length
323
NCBI Gene Symbol
Anxa3
Protein Name
O35639
Gene Name URL
Anxa3
Nucleotide Accession Number
NM_013470.2

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