MIOX ELISA Kit (Mouse)

CAT: 0247-OKEH05813-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05813-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Myo-inositol oxygenase
Gene Aliases
0610009I10Rik; AI314022; Al; aldehyde reductase (aldose reductase) -like 6; aldehyde reductase 6, renal; aldehyde reductase-like 6; Aldrl6; C85427; inositol oxygenase; MI oxygenase; Myo-inositol oxygenase; renal-specific oxido-reducatse; renal-specific oxidoreductase; RSOR.
Gene ID
56727
Accession Number
NP_064361.2
Reactivity
Mouse, Mus musculus
Target
Inositol oxygenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Miox ELISA Kit (Mouse) (OKEH05813) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Miox has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Miox is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Miox captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.8%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Inositol oxygenase
Protein Length
285
NCBI Gene Symbol
Miox
Protein Name
Q9QXN5
Gene Name URL
Miox
Nucleotide Accession Number
NM_019977.2

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