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LAP3 ELISA Kit (Mouse)

CAT: 0247-OKEH05804-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05804-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Leucine aminopeptidase 3
Gene Aliases
2410015L10Rik; AA410100; cysteinylglycine-S-conjugate dipeptidase; cytosol aminopeptidase; Lap; LAP-3; Lapep; Leucine aminopeptidase 3; leucyl aminopeptidase; Pe; Pep; Pep7; Pep-7; Peps; Pep-S; peptidase 7; peptidase S; proline aminopeptidase; prolyl aminopeptidase.
Gene ID
66988
Accession Number
NP_077754.3
Reactivity
Mouse, Mus musculus
Target
Cytosol aminopeptidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lap3 ELISA Kit (Mouse) (OKEH05804) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lap3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lap3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lap3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cytosol aminopeptidase
Protein Length
519
NCBI Gene Symbol
Lap3
Protein Name
Q9CPY7
Gene Name URL
Lap3
Nucleotide Accession Number
NM_024434.6

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