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AQP2 ELISA Kit (Mouse)

CAT: 0247-OKEH05796-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05796-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Aquaporin 2
Gene Aliases
ADH water channel; AQP-2; AQP-CD; aquaporin-2; aquaporin-CD; collecting duct water channel protein; cph; jpk; water channel protein for renal collecting duct; WCH-CD.
Gene ID
11827
Accession Number
NP_033829.3
Reactivity
Mouse, Mus musculus
Target
Aquaporin-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Aqp2 ELISA Kit (Mouse) (OKEH05796) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aqp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Aqp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aqp2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 107%
Sensitivity
0.323 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Aquaporin-2
Protein Length
271
NCBI Gene Symbol
Aqp2
Protein Name
P56402
Gene Name URL
Aqp2
Nucleotide Accession Number
NM_009699.3

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