CD28 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  CD28 antigen

• Gene Aliases:

  T-cell-specific surface glycoprotein CD28.

• Gene ID:

  12487

• Accession Number:

  NP_031668.3

• Reactivity:

  Mouse, Mus musculus

• Target:

  T-cell-specific surface glycoprotein CD28

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Cd28 ELISA Kit (Mouse) (OKEH05786) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd28 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cd28 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd28 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.625-40ng/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 93.7%

• Sensitivity:

  0.325 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse T-cell-specific surface glycoprotein CD28

• Protein Length:

  218

• NCBI Gene Symbol:

  Cd28

• Protein Name:

  P31041

• Gene Name URL:

  Cd28

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_007642.4