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PTGFR ELISA Kit (Mouse)

CAT: 0247-OKEH05776-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05776-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Prostaglandin F receptor
Gene Aliases
AI957154; F; fp; PG; PGF; PGF receptor; PGF2-alpha receptor; prostaglandin F2alpha receptor; prostaglandin F2-alpha receptor; prostanoid FP receptor.
Gene ID
19220
Accession Number
NP_032992.1
Reactivity
Mouse, Mus musculus
Target
Prostaglandin F2-alpha receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ptgfr ELISA Kit (Mouse) (OKEH05776) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptgfr has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ptgfr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptgfr captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.1%
Sensitivity
0.331 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Prostaglandin F2-alpha receptor
Protein Length
366
NCBI Gene Symbol
Ptgfr
Protein Name
P43117
Gene Name URL
Ptgfr
Nucleotide Accession Number
NM_008966.3

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