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MTBP ELISA Kit (Mouse)

CAT: 0247-OKEH05640-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05640-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Mdm2, transformed 3T3 cell double minute p53 binding protein
Gene Aliases
AI429604; MDM2B; mdm2-binding protein; MDM2BP; mMTBP.
Gene ID
105837
Accession Number
NP_001161722.1
Reactivity
Mouse, Mus musculus
Target
Mdm2-binding protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mtbp ELISA Kit (Mouse) (OKEH05640) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mtbp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mtbp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mtbp captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.4%
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Mdm2-binding protein
Protein Length
894
NCBI Gene Symbol
Mtbp
Protein Name
Q8BJS8
Gene Name URL
Mtbp
Nucleotide Accession Number
NM_001168250.1

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